The effect of photodynamic therapy on Aggregatibacter actinomycetemcomitans attached to surface-modified titanium
조경원, 이시영, 장범석, 엄흥식, 이재관,
소속 상세정보
조경원 ( Cho Kyung-Won ) - Gangneung-Wonju National University College of Dentistry Department of Periodontology
이시영 ( Lee Si-Young ) - Gangneung-Wonju National University College of Dentistry Department of Oral Microbiology
장범석 ( Chang Beom-Seok ) - Gangneung-Wonju National University College of Dentistry Department of Periodontology
엄흥식 ( Um Heung-Sik ) - Gangneung-Wonju National University College of Dentistry Department of Periodontology
이재관 ( Lee Jae-Kwan ) - Gangneung-Wonju National University College of Dentistry Department of Periodontology
KMID : 0363020150450020038
Abstract
Purpose: The purpose of this study was to evaluate the effect of photodynamic therapy (PDT) using erythrosine and a green light emitting diode (LED) light source on biofilms of Aggregatibacter actinomycetemcomitans attached to resorbable blasted media (RBM) and sandblasted, large-grit, acid-etched (SLA) titanium surfaces in vitro.
Methods: RBM and SLA disks were subdivided into four groups, including one control group and three test groups (referred to as E0, E30, E60), in order to evaluate the effect of PDT on each surface. The E0 group was put into 500 μL of 20 μM erythrosine for 60 seconds without irradiation, the E30 group was put into erythrosine for 60 seconds and was then irradiated with a LED for 30 seconds, and the E60 group was put into erythrosine for 60 seconds and then irradiated with a LED for 60 seconds. After PDT, sonication was performed in order to detach the bacteria, the plates were incubated under anaerobic conditions on brucella blood agar plates for 72 hours at 37℃, and the number of colony-forming units (CFUs) was determined.
Results: Significant differences were found between the control group and the E30 and E60 groups (P<0.05). A significantly lower quantity of CFU/mL was found in the E30 and E60 groups on both titanium disk surfaces. In confocal scanning laser microscopy images, increased bacterial death was observed when disks were irradiated for a longer period of time.
Conclusions: These findings suggest that PDT using erythrosine and a green LED is effective in reducing the viability of A. actinomycetemcomitans attached to surface-modified titanium in vitro.
키워드
Bacterial viability ; Erythrosine ; Photodynamic therapy ; Titanium
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